Monograph

Working with rhizobia

Date released
04 May 2016
Publication Code
MN173
Authors

Ricardo Silva Araujo, Julie Ardley, Robert Abaidoo, Abdullahi Bala, Roz Deaker, Michael Dilworth, Greg Gemell, Ken Giller, Elizabeth Hartley, David Herridge, John Howleson, Marlangela Hungrla, Nancy Karanja, Karunakaran Ramakrishnan, Vanessa Melino, Sofle De Meyer, Graham O'Hara, Philip Poole, Wayne Reeve, Janet Sprent, Jason Terpolilli, Ravl Tlwarl, Paul Woomer, Ron Yates and Jerry Zilli Howieson J.G. and Dilworth M.J. (Eds.).

Overview

 

This manual brings together state-of-the-art methods for the study of root-nodule bacteria, both in the free-living state and in symbiosis with legumes.

In each chapter, the manual introduces a topic and provides guidance on how study of the symbiosis might best be tackled.

Detailed descriptions of the protocols that need to be followed, potential problems and pitfalls are provided. Topics covered include acquiring, recognising, growing and storing rhizobia, experimenting with strains in the laboratory, glasshouse and field, and applying contemporary molecular and genetic methodologies to assist in the study of rhizobia.