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Crops

Optimisation of the single drop genomics assay to detect food pathogens

Project Code: CIM/2014/027
Program: Crops
Budget:
A$173,455
Research Program Manager: Dr. Eric Huttner
Project Leader: Jimmy Botella - University of Queensland
Duration:
JUN 2014
MAY 2015
Project Status: Concluded
Key partners
Cambodia Agricultural Research and Development Institute
Department of Primary Industries
National Agriculture and Forestry Research Institute
DOCUMENTS

Overview

This SRA aimed to optimise Single-Drop Genomics (SDG) technology to detect food borne pathogens.

Nucleic acid point-of-care (POC) bioassays that can be performed on-site with minimal equipment, rapidly and at low cost are in high demand. Agriculture is one area that can benefit from the use of low cost on-site assays.

This project is also listed as HORT/2014/027.

 

Outcomes

On-site, quick and low-cost pathogen detection is the holy grail of disease diagnostics. Our research team has developed a comprehensive, field-ready technology for on-site pathogenic nucleic acid detection named Single-Drop Genomics (SDG).  SDG combines a simple yet accurate on-site sampling process with a robust isothermal amplification and a novel naked-eye evaluation providing Yes/No results.

In this project we have optimised the SDG technology to detect food borne pathogens. We have also performed extensive consultations with multiple stakeholders in Cambodia and Laos PDR about the possible applications of the technology in both countries. SDG has been identified by local officials as being a potential ‘game changer’ capable of delivering strong benefits for different agricultural sectors.